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Autor:  

Isik, Sonnur

Titel:  

Electrochemical detection of nitric oxide from living cells


Dissertation 
URN:  urn:nbn:de:hbz:294-15819
URL:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/IsikSonnur/diss.pdf
Format:  application/pdf (6.4 M)
Kommentar:  Ruhr-Universität Bochum, Fakultät für Chemie. Tag der mündlichen Prüfung: 2006-02-03

Inhaltsverzeichnis
Datei:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/IsikSonnur/Inhaltsverzeichnis.pdf
Format:  application/pdf (50.5 k)

Zusammenfassung
Datei:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/IsikSonnur/Zusammenfassung.pdf
Format:  application/pdf (40.4 k)

Schlagworte:  Rasterelektrochemisches Mikroskop; Biosensor; Elektrochemie; Glutamate; Neurotransmitter

Inhalt der Arbeit: 

Two different types of NO-sensor were developed. The first type of NO-sensor developed was based on the electrochemical entrapment of Ni-TmPyP within a polymer matrix formed by anodic electrodeposition paint on the surface of Pt electrodes. The second type of NO-sensor was developed by polymerizing a layer of Ni-TSPc on a Pt surface followed by layer of Nafion®. The chemical modifications on both types of electrode developed were successfully applied for the sensitive determination of nitric oxide. In the measurement of NO release from living cells, the positioning of the NO-sensor plays an important role. Hence, different procedures were developed for accurate and precise detection of NO. Simultaneous detection of NO and glutamate from cells at a known distance was successfully achieved. Finally, single cell measurement of NO release was achieved with the combination of a developed highly sensitive vibrational disk-shaped NO-sensor and constant distance mode SECM at a sub-μm distance.


Angaben des Autors:
E-Mail:  sonnur.isik@rub.de
Homepage: 
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