Viruses are a major cause of waterborne and water related diseases. A few viral particles can be found in surface water, so enrichment steps are required prior to the analysis of water samples. The virus adsorption elution method was optimized for
enterovirus (EV), human adenovirus (HAdV), human polyomavirus (HPyV), norovirus (NoV) and somatic coliphages (SOMCPH). The results revealed that high salt-alkaline solution of phosphate buffer with 0.1%Trition X-100 is the buffer of choice for
simultaneous recovery of viruses under study. Since it is not practical to monitor all viruses that may contaminate water, virus indicator of water contamination is needed. Accordingly, different viruses were proposed as indicators of water contamination
such as EV, HAdV, HPyV, human bocavirus, NoV, rotavirus, Torque teno virus, human picobirnavirus, pepper mild mottle virus (PMMoV) and SOMCPH. This work showed that HAdV, HPyV and PMMoV could be promising indicators of surface water contamination.