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Autor:  

El-Mohsnawy, Eithar Abdel Rashid

Titel:  

Evaluierung von monomerem und trimerem Photosystem 1 in nativen Systemen sowie in semiartifiziellen Systemen zur Biowasserstofferzeugung

übersetzter Titel:  

Evaluation of monomeric and trimeric PS1 in native systems and in semiartificial systems for biohydrogen production


Dissertation 
URN:  urn:nbn:de:hbz:294-20186
URL:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/ElMohsnawyEitharAbdelRashid/diss.pdf
Format:  application/pdf (4.4 M)
Kommentar:  Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie. Tag der mündlichen Prüfung: 2007-08-30

Inhaltsverzeichnis
Datei:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/ElMohsnawyEitharAbdelRashid/Inhaltsverzeichnis.pdf
Format:  application/pdf (124.3 k)

Zusammenfassung
Datei:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/ElMohsnawyEitharAbdelRashid/Zusammenfassung.pdf
Format:  application/pdf (98.6 k)

Abstract
Datei:  http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/ElMohsnawyEitharAbdelRashid/Abstract.pdf
Format:  application/pdf (96.6 k)

Schlagworte:  Photosystem; Zirkulardichroismus; Lineardichroismus; Photostrom; Photostabilität

Inhalt der Arbeit: 

Purification of highly active trimeric PS1 was optimized and speeded up from 32 to 15 hours, and new and mild methods were developed for the qualitative and quantitative production of monomeric PS1. This enabled for a detailed analysis in comparison with the trimeric form in terms of pigment/cofactor contents, subunit composition, photosynthetic electron transport and photocurrent of immobilized PS1. Both complexes showed almost identical spectroscopic characteristics of LD-spectra, redox potential, Chl a/P700 ratio, carotenoid contents, subunit composition and activity. Modeling of kinetics measurements (time resolved fluorescence in the lower ps range) indicated the same origin of the long wavelength chlorophylls (LWCs) in both complexes.
Optimization of photocurrent measurements of PS1, which had been immobilized on a gold surfaces by a His-tag, showed the higher efficiency of the artificial electron donor PMS in comparison with the native Cyt c6.


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